Coding

Part:BBa_K4812021:Design

Designed by: Sanshika Garg   Group: iGEM23_NYU-Abu-Dhabi   (2023-10-10)


SclB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 587
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 587
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 587
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 587
    Illegal AgeI site found at 454
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 898


Design Notes

repeat region: caaaacaa aacaaaacaa aacaaaa


Source

Streptococcus pyogenes found through GenBank https://www.ncbi.nlm.nih.gov/nuccore/AJ301806

References

https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-147-2-419